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Image Search Results
Journal: Analytical Cellular Pathology
Article Title: Detection of The TNFSF Members BAFF, APRIL, TWEAK and Their Receptors in Normal Kidney and Renal Cell Carcinomas
doi: 10.1155/2011/108631
Figure Lengend Snippet: Fig. 1. Representative staining of BAFF, APRIL, TWEAK and their receptors (BAFFR, TACI, BCMA and Fn14) in normal renal tissue. Areas of a normal kidney are stained for BAFF, APRIL. TWEAK and their receptors (BAFFR, TACI, BCMA and Fn14), according to the method described in Materials and methods. The same case is presented in the different panels. Bar: 200 m. Ahigher magnification of APRIL immunostaining is also presented.
Article Snippet: Specific antibodies used were: APRIL (hAprily-8 mouse monoclonal antibody, 1/100 dilution, ALX-804-149, Alexis Co, Lausanne, Switzerland), BAFF (ALX-804-131 monoclonal antibody/ Buffy-2 clone, dilution 1/100, Alexis), TWEAK (sc12405 goat polyclonal antibody, 1/100 dilution, Santa Cruz Biotechnology, CA, USA), BCMA (Vicky-1 rat monoclonal antibody, 1/100 dilution, ALX-804-151, Alexis), TACI (IMG-249 rabbit polyclonal antibody, 1/150 dilution, Imgenex, San Diego, CA, USA), BAFF-R (goat polyclonal, AF1162, dilution 1/100, R&D Systems, Minneapolis, MN, USA) and
Techniques: Staining, Immunostaining
Journal: Analytical Cellular Pathology
Article Title: Detection of The TNFSF Members BAFF, APRIL, TWEAK and Their Receptors in Normal Kidney and Renal Cell Carcinomas
doi: 10.1155/2011/108631
Figure Lengend Snippet: Fig. 2. Detection of BAFF, APRIL, TWEAK and their receptors (BAFFR, TACI, BCMA and Fn14) in selected cases of RCC-CT. TNFSF ligands and receptors were assayed as described in Materials and methods. Cases of Fuhrman Grades 1–4 are presented. Bar = 100 m.
Article Snippet: Specific antibodies used were: APRIL (hAprily-8 mouse monoclonal antibody, 1/100 dilution, ALX-804-149, Alexis Co, Lausanne, Switzerland), BAFF (ALX-804-131 monoclonal antibody/ Buffy-2 clone, dilution 1/100, Alexis), TWEAK (sc12405 goat polyclonal antibody, 1/100 dilution, Santa Cruz Biotechnology, CA, USA), BCMA (Vicky-1 rat monoclonal antibody, 1/100 dilution, ALX-804-151, Alexis), TACI (IMG-249 rabbit polyclonal antibody, 1/150 dilution, Imgenex, San Diego, CA, USA), BAFF-R (goat polyclonal, AF1162, dilution 1/100, R&D Systems, Minneapolis, MN, USA) and
Techniques:
Journal: Analytical Cellular Pathology
Article Title: Detection of The TNFSF Members BAFF, APRIL, TWEAK and Their Receptors in Normal Kidney and Renal Cell Carcinomas
doi: 10.1155/2011/108631
Figure Lengend Snippet: Fig. 4. Detection of normalized mRNA expression of BAFF, APRIL, TWEAK and their receptors (BAFFR, TACI, BCMA and Fn14) in renal cell carcinoma, by qRT-PCR. A) Normalized qRT-PCR differences between cancerous and normal renal tissue (1 = normal). Table presents means and statistical values of qRT-PCR difference of expression of the TNFSF ligands and receptors in fresh-frozen tissue samples of a subset of cases (n = 14, presented in bold in Supplementary Table 1). B) Expression of TNFSF ligands and receptors in three published arrays (accession numbers are given in the legend).
Article Snippet: Specific antibodies used were: APRIL (hAprily-8 mouse monoclonal antibody, 1/100 dilution, ALX-804-149, Alexis Co, Lausanne, Switzerland), BAFF (ALX-804-131 monoclonal antibody/ Buffy-2 clone, dilution 1/100, Alexis), TWEAK (sc12405 goat polyclonal antibody, 1/100 dilution, Santa Cruz Biotechnology, CA, USA), BCMA (Vicky-1 rat monoclonal antibody, 1/100 dilution, ALX-804-151, Alexis), TACI (IMG-249 rabbit polyclonal antibody, 1/150 dilution, Imgenex, San Diego, CA, USA), BAFF-R (goat polyclonal, AF1162, dilution 1/100, R&D Systems, Minneapolis, MN, USA) and
Techniques: Expressing, Quantitative RT-PCR